EFEITO DA SUPLEMENTAÇÃO DE GLICOSE EM CÉLULAS EPITELIAIS BRÔNQUICAS HUMANAS INFECTADAS COM Cryptococcus neoformans

Autores

  • Roberta Pereira Soares Emrich Universidade Federal do Triângulo Mineiro
  • Giovanna Ferreira Bueno Universidade Federal do Triângulo Mineiro
  • Wanessa Maria dos Santos Universidade Federal do Triângulo Mineiro
  • Aline Beatriz Mahler Pereira Universidade Federal do Triângulo Mineiro
  • Leonardo Euripedes de Andrade e Silva Universidade Federal do Triângulo Mineiro
  • oão Batista Camargo Neto Faculdade de Medicina de Ribeirão Preto
  • Paulo Roberto da Silva Universidade Federal do Triângulo Mineiro
  • Mario Leon Silva-Vergara Universidade Federal do Triângulo Mineiro
  • Alexandre Paula Rogerio Universidade Federal do Triângulo Mineiro

DOI:

https://doi.org/10.18554/acbiobras.v8i2.8766

Palavras-chave:

células epiteliais brônquicas, Cryptococcus neoformans, citocinas , glicose

Resumo

A hiperglicemia pode causar deficiências nas respostas imune inata e adaptativa, além de aumentar a suscetibilidade a infecções fúngicas invasivas, como a criptococose. Avaliamos os efeitos da interação entre Cryptococcus neoformans (MOI 100) nas células BEAS-2B suplementadas com glicose (22,5 and 35 mM) após 24 horas de estímulo. Não foram detectados efeitos citotóxicos nas células estimuladas com C. neoformans e/ou suplementadas com glicose. Em comparação ao controle, C. neoformans aumentou a produção de IL-6 e IL-8, bem como a ativação de NF-κB, ERK1/2 e STAT3 nas células BEAS-2B. A suplementação de glicose promoveu efeitos pró-inflamatórios ao aumentar a produção de IL-6 e a ativação de fosfo-STAT3 nas células epiteliais brônquicas infectadas com C. neoformans, quando comparadas às apenas infectadas. Além disso, a suplementação de glicose promoveu efeitos anti-inflamatórios ao reduzir a produção de IL-8 e a fosforilação de ERK1/2. Ademais, a suplementação de glicose aumentou a internalização de C. neoformans pelas células, reduziu a porcentagem de fungos aderidos à superfície celular e diminuiu seu crescimento. Esses resultados demonstram que o aumento de glicose modula a ativação da resposta imune inata das células epiteliais brônquicas modulando a infecção criptocócica.

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31-10-2025

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v. 8 n. 2 (2025)

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EFEITO DA SUPLEMENTAÇÃO DE GLICOSE EM CÉLULAS EPITELIAIS BRÔNQUICAS HUMANAS INFECTADAS COM Cryptococcus neoformans. Acta Biologica Brasiliensia, [S. l.], v. 8, n. 2, p. 367–387, 2025. DOI: 10.18554/acbiobras.v8i2.8766. Disponível em: https://seer.uftm.edu.br/revistaeletronica/index.php/acbioabras/article/view/8766. Acesso em: 13 dez. 2025.