EFEITOS IN VITRO DOS NANOPLÁSTICOS DE POLIESTIRENO SOBRE A MOTILIDADE E A ATIVIDADE MITOCONDRIAL DE ESPERMATOZOIDES HUMANOS
DOI:
https://doi.org/10.18554/acbiobras.v8i2.8765Keywords:
Plastic, gamete, mitochondria, reproductionAbstract
É estimado que cerca de 350 milhões de toneladas de plástico se tornam resíduos, por meio de processos físicos e químicos, formando microplásticos e nanoplásticos. Considerando a crescente produção global de plásticos e seus potenciais impactos ambientais e à saúde humana, o presente estudo avaliou os efeitos do nanoplástico de poliestireno (NP-PS) sobre parâmetros espermáticos humanos in vitro. Espermatozoides de voluntários saudáveis (n=7) foram obtidos e incluídos apenas se a amostra se enquadrasse dentro dos padrões de normalidade estabelecidos pela Organização Mundial da Saúde. As células foram isoladas por swim-up em meio BWW e expostas a NP-PS em concentrações de 2, 20 e 200 μg/mL, além de grupo controle, por 30 e 120 minutos. A motilidade e atividade mitocondrial foram avaliadas. Os resultados mostraram que concentrações intermediárias e altas reduziram significativamente a motilidade e a funcionalidade mitocondrial, com efeitos dependentes de concentração e tempo. Estes achados indicam que NP-PS pode comprometer funções essenciais à fertilidade masculina, reforçando a necessidade de estudos adicionais sobre seus mecanismos e riscos à saúde reprodutiva.
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