ANÁLISE COMPARATIVA ENTRE A AÇÃO ANTIMICROBIANA DO EXTRATO DO Syzygium cumini E O CIPROFLOXACINO EM AMOSTRAS DE Staphylococcus aureus
DOI:
https://doi.org/10.18554/acbiobras.v7i2.7693Keywords:
Syzygium cumini, Staphylococcus aureus, Antibiogram test, Inhibition Halo, Secondary MetabolitesAbstract
Staphylococcus aureus is a bacterium that has been widely studied for its resistance and involvement in nosocomial infections. On the other hand, Syzygium cumini is a plant that is widely available in the state of Bahia, and its secondary metabolites have already been classified in the literature as having potential bactericidal activity. The aim of this study was to investigate the antibacterial properties of S. cumini against S. aureus, using the ethanolic extract and subjecting it to the antibiogram test, with the inhibition halo of ciprofloxacin as a reference for the size of its inhibition, as a control group. Secondary metabolites were extracted from the alcoholic macerated crude extract, which was then tested on an ATCC sample of S. aureus using the antibiogram. After extraction, a yield of 12.8% was obtained from the final material. It was observed that the extract led to the formation of significant inhibition halos at concentrations of 100%, 95%, 90% and 80%, with diameters ranging from approximately 12 mm to 15 mm between the sample groups. These results suggest that the extract is effective against S. aureus, highlighting its antimicrobial potential.
References
Organização Pan-Americana de Saúde - OPAS. Trabalhando juntos para combater a resistência aos antimicrobianos, 2022. Disponível em: https://www.paho.org/pt/juntos-combaterresistencia-antimicrobianos#section-3.
Amin AN, Cerceo EA, Deitelzweig SB, Pile JC, Rosenberg DJ, Sherman BMHospitalist Perspective on the Treatment of Skin and Soft Tissue Infections. Mayo Clin Proc, 89(10), 51-1436, 2014. https://doi.org/10.1016/j.mayocp.2014.04.018.
Dalfino L, Bruno F, Colizza S, Concia E, Novelli A, Rebecchi F, Spandonaro F, Alato C. Cost of care and antibiotic prescribing attitudes for community-acquired complicated intra-abdominal infections in Italy: a retrospective study.World J Emerg Surg, 9, 39, 2014. https://doi.org/10.1186/1749-7922-9-39.
Schmidt C, Köhler F, Kräplin T, Hartmann M, Lerch MM, Stallmach A. [Does the hospital cost of care differ for inflammatory bowel disease patients with or without gastrointestinal infections? A case-control study], 52(7), 8-648, 2014. https://doi.org/10.1055/s-0034-1365956.
Ondusko DS, Nolt D. Staphylococcus aureus. Pediatr, 39(6), 289-298, 2018. https://doi.org/10.1542/pir.2017-0224.
Rasheed NA, Hussein NR. Staphylococcus aureus: an overview of discovery, characteristics, epidemiology, virulence factors and antimicrobial sensitivity. European Journal of Molecular & Clinical Medicine, 8(3), 1160-1183, 2021. Disponível em: https://www.academia.edu/86023572/Staphylococcus_aureus_An_Overview_of_Discovery_Characteristics_Epidemiology_Virulence_Factors_and_Antimicrobial_Sensitivity_Short_Title_Methicillin_Resistant_Staphylococcus_aureus_An_overview?sm=b.
Macharete TC, Oliveira TLR, Guedes EM, Igari GF, Sant'Ana AC, Costa CR, Ferreira ALP, Nóuer SA, Cavalcante FS, Santos KRN. Staphylococcus aureus resistente à meticilina de colonização nasal de pacientes internados em UTIs de um hospital do Rio de Janeiro na pandemia de covid-19: aspectos associados à virulência e à tolerância aos saneantes. Braz J. Infect Dis., 27, 320, 2023. https://doi.org/10.1016/j.bjid.2023.103423.
Kolk TN, Buters TP, Krouwels L, Boltjes J, Kam KL, Wall HV, Alewijk DCJG, Munckhof EHA, Becker MJ, Feiss G, Florencia EF, Prens EPP, Moerland M, Burggraaf J, Rissmann R, Doorn MBA. Topical antimicrobial peptide omiganan recovers cutaneous dysbiosis but does not improve clinical symptoms in patients with mild to moderate atopic dermatitis in a phase 2 randomized controlled trial. Journal of the American Academy of Dermatology, 86(4), 854-862, 2022. https://doi.org/10.1016/j.jaad.2020.08.132.
Taylor AT, Unakal CG. Infecção por Staphylococcus aureus. Statpeaperls, 2023. Disponível em: https://www.ncbi.nlm.nih.gov/books/NBK441868/.
Cardoso AR, Carneiro LPT, Cabral-Miranda G, Bachmann MF, Sales MGF. Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection. Chemical Engineering Journal, 409, 128135, 2021. https://doi.org/10.1016/j.cej.2020.128135.
Zumla A. Mandell; Douglas; Bennett's. Principles and Practice of Infectious Diseases. Rev. Elsevier/Saunders, ed. 9, 2020. https://doi.org/10.1016/S1473-3099(10)70089-X.
Sampaio OS, Sancho LG, Lago RF. Implementação da nova regulamentação para prescrição e dispensação de antimicrobianos: possibilidades e desafios. Caderno Saúde Coletiva, 26(1), 2018. https://doi.org/10.1590/1414-462X201800010185.
Steiner F, Zuffo AM, Zoz T. Physical characterization of fruits and seeds of Jambolan [Syzygium cumini (L.) Skeels] (Myrtaceae). Acta Iguazu, 6(3), 2017. Disponível em: https://e-revista.unioeste.br/index.php/actaiguazu/article/view/16551.
Oliveira MS, Oliveira ES, Santos JV, Costa NB, Cardoso DT, Saboia CS, Ferreira MBC, Tales AM, Mouchrek AN. Estudo do perfil fitoquímico e avaliação da atividade antioxidante do extrato vegetal de Syzygium cumini. Research, Society and Development, 11(9), p. e37911931808-e37911931808, 2022. https://doi.org/10.33448/rsd-v11i9.31808.
Sousa MM, Lima RMT, Lima A, Reis AC, Cavalcante AACM, Sattler JAG, Almeida-Murandian LB, Neto JSL, Moreira-Araujo RSR, Nogueira NN. Antioxidant action and enzyme activity modulation by bioaccessible polyphenols from jambolan (Syzygium cumini (L.) Skeels). Food Chemistry. 363, 130353, jun. 2021. https://doi.org/10.1016/j.foodchem.2021.130353.
Pessoa RLD. Farmácia na atenção e assistência à saúde. Atenas, 4, 1-29, 2021. Disponível em: https://atenaeditora.com.br/catalogo/ebook/farmacia-na-atencao-e-assistencia-a-saude-4.
Soares NP, Santos PL, Vieira VS, Pimenta VSC, Araújo EG. Técnicas De Prospecção Fitoquímica E Sua Importâcia Para O Estudo De Biomoléculas Derivadas De Plantas. Enciclopédia Biosfera - Centro Científico Conhecer - Goiânia, 13(24), 1-20, 2016. https://doi.org/10.18677/EnciBio_2016B_094.
Gupta K, Singh SP, Manhar AK, Saikia D, Namsa ND, Konwar BK, Mndal M. Inhibition of Staphylococcus aureus and Pseudomonas aeruginosa Biofilm and Virulence by Active Fraction of Syzygium cumini (L.) Skeels Leaf Extract: In-Vitro and In Silico Studies. Indian J Microbiol, 59, 13–21, 2019. https://doi.org/10.1007/s12088-018-0770-9.
Hornes MO, Pinheiro PN. Caracterização físico-química e sensorial de fermentado de jambolão (Syzygium cumini) produzido a partir do mosto da maceração da polpa e por extração a vapor. Revista Brasileira de Tecnologia Agroindustrial, 15(2), 18, 2021. doi: https://doi.org/10.3895/rbta.v15n1.12725.
Comitê Brasileiro de Teste de Sensibilidade aos Antimicrobianos (BrCat). Método de disco-difusão BrCAST-EUCAST. RJ, 15 abr 2024, 12:1-29. Disponível em: https://brcast.org.br/wp-content/uploads/2022/09/Metodo-disco-difusao-BrCAST-15-04-2024.pdf.
Comitê Brasileiro de Teste de Sensibilidade aos Antimicrobianos (BrCast). Tabelas de pontos de corte para interpretação de CIMs e diâmetros de halos. RJ, 15 mar 2023, 13:1-90. Disponível em: https://brcast.org.br/documentos/documentos-3/.
Haque R, Sumiya MK, Sakib N, Sarkar OS, Siddique TTI, Hossain S, Islam A, Parvez AK, Talukder AA, Dey SK. Antimicrobial Activity of Jambul (Syzygium cumini) Fruit Extract on Enteric Pathogenic Bacteria. Rev. Scientific Research, 7(3), 10, 2017. https://doi.org/10.4236/aim.2017.73016.
Mendonça AT, Carvalho AR, Ferreira MC, Júnior MCR. A utilização dos extratos hidroalcoólico e alcoólico de Eugenia uniflora L. como agende antibacteriano. Vale, 14(1), 2016, 826-833. http://dx.doi.org/10.5892/ruvrd.v14i1.3019.
Santos CA, Almeida FA, Quecán BXV, Pereira PAP, Gandra KMB, Cunha LR, Pinto UM. Bioactive Properties of Syzygium cumini (L.) Skeels Pulp and Seed Phenolic Extracts. Front Microbiol, 27(11), 2020. https://doi.org/10.3389/fmicb.2020.00990.
Veber J, Petrini LA, Andrade LB, Siviero J. Determinação dos compostos fenólicos e da capacidade antioxidante de extratos aquosos e etanólicos de Jambolão (Syzygium cumini L.). Rev. bras. plantas med., 17(2), 2015. https://doi.org/10.1590/1983-084X/12_181.
Singh JP, Kaur A, Singh N, Nim L, Shevkani K, Kaur H, Arora DS. In vitro antioxidant and antimicrobial properties of jambolan (Syzygium cumini) fruit polyphenols. Food Science and Technology, 65, 1025-1030, 2016. https://doi.org/10.1016/j.lwt.2015.09.038.
Bari TSS, Tayyab M, Anjum, AA, Mehmood T. GC-MS Bioactives Profiling, Antibacterial and Cytotoxic Potential of Jamun (Syzygium cumini L.) Extracts Against Food-Borne Pathogen Salmonella enteritidis. Pharma Chem, 57, 1586–1592, 2024. https://doi.org/10.1007/s11094-024-03052-x.
