Microrganismos endofíticos como fonte de compostos de interesse medicinal – uma breve revisão

Autores

DOI:

https://doi.org/10.18554/rbcti.v5i1.3600

Palavras-chave:

Atividade biológica, Endofitismo, Plantas, Streptomicetos.

Resumo

Os microrganismos endofíticos, vivem no interior dos tecidos de plantas sem causar nenhum dano aparente ou sinal externo de infecção às mesmas e são pouco estudados. Esses residem, preferencialmente, nos espaços intercelulares mais do que nos espaços intracelulares dos tecidos vegetais e já foram detectados em plantas tropicais, temperadas e de florestas boreais. Os microrganismos endofíticos apresentam importante papel nos ecossistemas influenciando na biodiversidade das comunidades e mediando as interações ecológicas. Além disso, podem atuar na defesa das plantas, diminuir os danos causados por agentes patógenos, insetos, acelerar a emersão de sementes, promover o equilíbrio da planta em condições adversas e aumentar o seu crescimento. Nesse trabalho encontra-se uma breve revisão de artigos científicos que abordam o amplo espectro de atividades biológicas que compostos produzidos por microrganismos endofíticos podem executar, reforçando a importância desses como fonte de compostos de interesse medicinal.

Biografia do Autor

Douglas Fabiano Villas Boas, PMPIT/UFTM

Mestre em Inovação Tecnológica

Geoffroy Roger Pointer Malpass, Universidade Federal do Triângulo Mineiro (UFTM)

Cursei a minha graduação em Química (BSc. Honours Chemistry - 1998) na University of Wales (Grá-Bretanha) com um estágio de um ano, na comunidade européia - Universidade de Lisboa (1996-1997). Em seguida entrei no curso de Mestrado em Química Ambiental (MSc. Environmental Chemistry) sob a orientação do Prof. Dr Maher Kalaji, sendo aprovado com mérito em 1999. Já o doutoramento foi feito no Instituto de Química de São Carlos (USP), com o título obtido em 2004. Desde a graduação minha pesquisa concentrou-se na área de físico química, particularmente na eletroquímica, voltado à solução de problemas ambientais. De forma geral, trata-se de estudos aplicados, mas, com um forte componente fundamental. No mestrado, foi realizado o estudo de remoção de Ni(II) por métodos eletroquímicos de efluente simulados. Apesar de ter enfoque ambiental o projeto destacou-se pela aplicação de métodos físicos e físico-químicos para obter uma correlação entre os depósitos obtidos e as condições de reação empregadas. No doutorado, foi feita a preparação de vários eletrodos cerâmicos, sua subseqüente caracterização por vários métodos físicos e físico-químicos e, finalmente, a sua aplicação na oxidação de substâncias orgânicas em escala de bancada e piloto. O trabalho de pós-doutoramento teve como enfoque (vide publicações recentes) o tratamento eletroquímico de resíduos de pesticidas e efluentes industriais têxteis. Atualmente sou professor adjunto da Universidade Federal do Triângulo Mineiro (UFTM), atuando na área de química verde e eletroquímica ambiental.

Ana Claudia Granato, UFTM

Bacharelado em Química e Doutorado em Química Analítica

Referências

ALI, L. et al. Sorokiniol: a new enzymes inhibitory metabolite from fungal endophyte Bipolaris sorokiniana LK12. BMC Microbiology, v.16, p.1-9, 2016.

ALMEIDA, T. T. et al. Curvulin and spirostaphylotrichins R and U from extracts produced by two endophytic Bipolaris sp. associated to aquatic macrophytes with antileishmanial activity. Natural Product Research, p.1-8, 2017.

ALY, A. H. et al. NF kappa B inhibitors and antitrypanosomal metabolites from endophyticfungus Penicillium sp. isolated from Limonium tubiflorum. Bioorganic & Medicinal Chemistry, v.19, p. 414–421, 2011.

ALY, A. H. et al. Cytotoxic metabolites from the fungal endophyte Alternaria sp. and their subsequent detection in its host plant Polygonum senegalense. Journal of Natural Products, v. 71, p. 972–980, 2008.

ALSHAIBANI, M. et al. Isolation, Purification, and Characterization of Five Active Diketopiperazine Derivatives from Endophytic Streptomyces SUK 25 with Antimicrobial and Cytotoxic Activities. Journal of Microbiology and Biotechnology, v. 27, n. 7, p. 1249–1256, 2017.

AMATUZZI, R. F. et al. Fungos endofíticos para o biocontrole de Duponchelia fovealis (Zeller) (Lepidoptera: Crambidae). Brazilian Journal of Biology, v. 78, p. 429-435, 2018.

AMRANI, M. E. et al. Farinomalein derivatives from an unidentified endophytic fungusisolated from the mangrove plant Avicennia marina. Tetrahedron Letters, v. 53, p. 6721– 6724, 2012.

BAELIEY, B. A. et al. Antibiosis, mycoparasitism, and colonization success for endophytic Trichoderma isolates with biological control potential in Theobroma cacao. Biological Control, v. 46, p. 24-35, 2008.

BALDANI, J. I.et al. Characterization of Herbaspirillurn seropedicae gen. nov. sp. nov. a Root- Associated Nitrogen-Fixing Bacterium. International Journal of Systematic Bacteriology, v. 36, p. 86–93, 1986.

BARRAQUIO, W. L. et al. Isolation of endophytic diazotrophic bacteria from wetland rice. Plant Soil, v. 194, p. 139–149,1997.

BODDEY, R. M. et al. Endophytic nitrogen fixation in sugarcane: present knowledge and future applications. Plant Soil, v. 252, p. 139–149, 2003.

BOONMAN, N. et al. Acanthamoebicidal activity of Fusarium sp. T l a u 3, and endophytic fungus from Thunbergia laurifolia Lindl. Parasitology Research, v. 103, p. 1083– 1090, 2008.

CALCUL, L. et al. Screening Mangrove Endophytic Fungi for Antimalarial Natural Products. Marine Drugs, v. 11, p. 5036-5050, 2013.

CAMPOS, F. F. et al. Leishmanicidal metabolites from Cochliobolus sp., an endophytic fungus isolated from Piptadenia adiantoides (Fabaceae). PLoS Neglected Tropical Disease, v. 2, p.1-11, 2008.

CASTILLO, U. F. et al. Munumbicins e-4 and e-5: novel broad-spectrum antibiotics from streptomyces NRRL 3052. Fems Microbiology Letters, v. 255, p. 296–300, 2006.

CONTI, R. et al. Endophytic Actinobacteria from the Brazilian Medicinal Plant Lychnophora ericoides MART. and the Biological Potential of Their Secondary Metabolites. Chemistry &. Biodiversity, v. 13, p. 727 – 736, 2016.

COTA, B. B. et al. Altenusin, abiphenyl isolated from the endophytic fungus Alternaria sp., inhibits trypanothione reductase from Trypanosoma cruzi. Fems Microbiology Letters, v. 285, p. 177-182, 2008.

CHOWDHURY, N. S. et al. Cytotoxic Naphthoquinone and Azaanthraquinone Derivatives from an Endophytic Fusarium solani. Journal of Natural Products, v. 80, p. 1173–1177, 2017.

DAÍ, C. C. et al. The screening and identification of endophytic fungi from four species of family Euphorbiaceae and the strain sp. antibacterial activity. Journal of Nanjing Forestry University, v. 30, p. 79–83, 2006.

DINESH, R. et al. Endophytic actinobacteria: Diversity, secondary metabolism and mechanisms to unsilence biosynthetic gene clusters. Critical Reviews in Microbiology, v. 43, p. 546-566, 2017.

EZRA, D. et al. Coronamycins, peptide antibiotics produced by verticillate Streptomyces sp. (MSU-2110) endophitic on Monstera sp. Microbiology, v. 150, p. 785–793, 2004.

FONSECA, E. S. et al. The Microbiome of Eucalyptus Roots under Different Management Conditions and Its Potential for Biological Nitrogen Fixation. Microbial Ecology, v. 75, p. 183–191, 2018.

GASONI, L.; GURFINKEL, B. S. The endophyte Cladorrhinum foecundissimum in cotton roots: phosphorus uptake and host growth. Mycological Research, v. 101, p. 867–870, 1997.

GOS, F. M. W. R. et al. Antibacterial Activity of Endophytic Actinomycetes Isolated from the Medicinal Plant Vochysia divergens (Pantanal, Brazil). Frontiers of Microbiology, v. 6, p. 1-17, 2017.

GUO, B. et al. Cytonicacids A and B: novel tridepside inhibitors of hCMV protease from the endophytic fungus Cytonaema species. Journal of Natural Products, v. 63, p. 602– 604, 2000.

GUO, B. et al. Bioactive natural products from endophytes: a review. Applied Biochemistry and Microbiology, v. 44, p. 136–142, 2008.

HENDRY, S. J.; BODDY, L.; LONSDALE, D. Abiotic variables effect differential expression of latent infectuosin beech (Fagus sylvatica). New Phytologyst, v. 55, p. 449–460, 2002.

HIGGINBOTHAM, S. et al. Sloth hair as a novel source of fungi with potent anti- parasitic, anti-cancer and anti-bacterial bioactivity. PLOS ONE, v. 15, p. 845-849, 2014.

HUANG, W. Y. et al. Biodiversity of endophytic fungi associated with 29 traditional Chinesemedicinal plants. Fungal Diversity, v. 33, p. 61–75, 2008.

INAHASHI, Y. et al. Spoxazomicins A-C, novel antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460(T). Journal of Antibiotics, v. 64, p. 303-307, 2011.

ISHII, T. et al. Cytosporone S with antimicrobial activity, isolated from the fungus Trichoderma sp. FKI-6626. Bioorganic and Medicinal Chemistry Letters, v. 23, p .679– 681, 2013.

JINFENG, E. G. et al. Analysis of chemical constituents, antimicrobial and anticancer activities of dichloromethane extract of Sordariomycetes sp. endophytic fungi isolated from Strobilanthes crispus. World Journal of Microbiology and Biotechnology, v. 33, n.1, p. 1-19, 2017.

KIM, S. et al. Periconicins,two new fusicoccane diterpenes produced by an endophytic fungus Periconia sp. with antibacterial activity. Journal of Natural Products, v. 67, p. 448-450, 2004.

LI, J. Y. et al. Endophytic taxol-producing fungi from bald cypress, Taxodium distichum. Microbiology, v. 142, p. 2223–2226, 1996.

LOIRET, F. G. et al. A putative new endophytic nitrogen-fixing bacterium Pantoea sp. from sugarcane. Journal of Applied Microbiology, v. 97, p. 504–511, 2004.

LU, H. et al. New bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua. Plant Science, v. 151, p. 67–73, 2000.

MALINOWSKI, D. P. et al. Adaptations of endophyte-infected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance. Crop Science, v. 183, p. 53–60, 1999.

MCCARTY, R. M.; BANDARIAN, V. Biosynthesis of pirrolopyrimidines. Bioorganic Chemistry, v. 43, p. 15-25, 2012.

MORICCA, S.; RAGAZZI, A. Fungal endophytes in Mediterranean oak forests: a lesson from Discula quercina. Phytopathology, v. 98, p. 380–386, 2008.

NEWMAN, L. A.; REYNOLDS, C. M. Bacteria and phytoremediation: new uses for endophytic bacteria in plants. Trends in Biotechnology, v. 23, p. 6–8, 2005.

OKANE, I.; NAKAGIRI, A.; ITO, T. Endophytic fungi in leaves of ericaceous plants. Canadian Journal of Botanics, v. 76, p. 657–663, 1998.

PATIl, M. P. et al. Biological Activities and Identification of Bioactive Metabolite from Endophytic Aspergillus flavus L7 Isolated from Aegle marmelos. Current Microbiology, v. 71, p. 39–48, 2015.

PATLE, P. N. et al. Endophytes in plant system: Roles in growth promotion, mechanism and their potentiality in achieving agriculture sustainability. International Journal of Chemical Studies, v. 6, p. 270-274, 2018.

PERVEEN, I. et al. Isolation of anticancer and antimicrobial metabolites from Epicoccum nigrum; endophyte of Ferula sumbul. Microbial Pathogenesis, v. 110, p. 214-224, 2017.

PHOTITA, W. et al. Are some endophytes of Musa acuminata latent pathogens? Fungal Diversity, v. 16, p. 131–140, 2004.

PURI, S. C. et al. An endophytic fungus from nothapodytes foetida that produces camptothecin. Journal of Natural Products, v. 68, p. 1717–1719, 2005.

RAGHUKUMAR, C. Marine fungal biotechnology: an ecological perspective. Fungal Diversity, v. 31, p. 19–35, 2008.

ROSA, L. H. et al. Leishmanicidal, Trypanocidal, and Cytotoxic Activities of Endophytic Fungi Associated with Bioactive Plants In Brazil. Brazilian Journal of Microbiology, v. 41, p. 420–430, 2010.

RUBINI, M. R. et al. Diversity of endophytic fungal community of cacao (Theobroma cacao l.) and biological control of Crinipellis perniciosa, causal agent of witches' broom disease. International Journal of Biological Science, v. 1, p. 24–33, 2005.

RYAN, R. P. et al. Bacterial endophytes: recent developments and applications. FEMS Microbiology Letters, v. 278, p. 1–9, 2008.

SAIKKONEN, K. Forest structure and fungal endophytes. Fungal Biology Review, v. 21, p. 67-74, 2007.

SANDHIYA, G. S. et al. Endophitic colonization and in planta nitrogen fixation by a diazotrophic Serratia sp. in rice. Indian Experimental Bioogy, v. 43, p. 802- 807, 2005.

SAPPAPAN, R. et al. 11-Hydroxymonocerin from the plant endophytic fungus Exserohilum rostratum. Journal of Natural Products, v. 71, n.9, p. 1657–1659, 2008.

SCHULZ, B. et al. Screening strategies for obtaining novel, biologically active, fungal secondary metabolites from marine habitats. Botanica Marina, v. 51, p. 219–234, 2008.

SIEBER, T. J. Endophytic fungi in forest trees: are they mutualists? Fungal Biology Review, v. 21, p. 75-89, 2007.

SONG, Y. C. et al. Endophytic naphthopyrone metabolites are co-inhibitors of xanthine oxidase, sw1116 cell and some microbial growths. Fems Microbiology Letters, v. 241, p. 67–72, 2004.

STIERLE, A. et al. Taxol and taxane production by taxomy ces andreanae, an endophytic fungus of pacific yew. SCIENCE, v. 260, p. 214–216,1993.

STONE, J. K. Fine structure of latent infections of Rabdocline parkeri ondouglas-fir. Canadian Journal of Botany, v. 66, p. 45–54, 1987.

STONE, J. K.; POLISHOOK, J. D.; WHITE, J. F. Endophytic fungi. In: MUELLER, G. M.; BILLS, G.F.; WHITE, J. F. (eds.). Biodiversity of Fungi. Amsterdam: Elsevier, 2004. p. 241–270.

STROBEL, G. et al. Ispoestacin, an isobenzofuranone from Pestaloliopsis microspore possessing antifunfal and antioxidant activity. Phytochemistry, v. 60, p. 179-183, 2002.

STROBEL, G. et al. Natural products from endophytic microorganisms. Journal of Natural Products, v. 67, p. 257-268, 2004.

STROBEL, G. A. et al. Pestalopiopsis guepinii, a taxol producing endophite of wollemi pine, Wollemia nobilis. Australian Journal of Botany, v. 45, p. 1073–1082, 1997.

STROBEL, G. A. et al. Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiology, v. 142, p. 435–440, 1996.

STROBEL, G. A.; DAISY, B. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Research, v. 67, p. 491–502, 2003.

SUMARAH, M. W.et al. Measurement of a rugulosin-producing endophyte in white spruce seedling. Mycologia, v. 97, p. 770–776, 2005.

SURYANARAYANAN, T. S.; THENNARASAN, S. Temporal variation in endophyte assemblages of Plumeria rubra leaves. Fungal Diversity, v. 15, p. 197–204, 2004.

TAECHOWISAN, T.; CHAISAENG, S.; PHUTDHAWONG, W. S. Antibacterial, antioxidant and anticancer activities of biphenyls from Streptomyces sp. BO-07: an endophyte in Boesenbergia rotunda (L.) Mansf A. Food and Agricultural Immunology, v. 28, n. 6, p.1-17, 2017.

TAN, R. X.; ZOU, W. X. Endophytes: a rich source of functional metabolites. Natural Products Report, v. 18, p. 448–459, 2001.

TAYLOR, J. E.; HYDE, K. D.; JONES, E. Endophytic fungi associated with temperate palms Tchrachycarpus fortunei, within and outside its natural geographic range. New Phytology, v. 42, p. 335–346, 1999.

TEJESVI, M. V. et al. New hopes from endophitic fungal secondary metabolites. Boletim de la Sociedad de Química de México, v. 1, p. 19-26, 2007.

VAN AKEN, B.; YOON, J. M.; SCHNOOR, J. L. Biodegradation of nitro-substituted explosives 2,4,6-trinitrotoluene, hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5-tetrazocine by a phytosymbiotic methylobacterium sp. associated with poplar tissues (populus deltoides × nigra dn34). Applied Environmental Microbiology, v. 70, p. 508–517, 2004.

VURUKONDA, S. S. K. P. et al. Plant Growth Promoting and Biocontrol Activity of Streptomyces spp. as Endophytes. International Journal of Molecular Science, v. 19, p. 952-978, 2018.

WAGENAAR, M. et al. three new cytochalasins produced by an endophytic fungus in the genus rhinocladiella. Journal of Natural Products, v. 63, p. 1692–1695, 2000.

WALL, M. E. et al. Plant antitumor agents. I. The isolation and structure of camptothecin, a novel alkaloidal leukemia and tumor inhibitor from Camptotheca accuminata. Journal of the American Chemical Society, v. 88, p. 3888–3890, 1966.

WANG, J. F. Taxol from Tubercularia sp. strain tf5, an endophytic fungus of taxus mairei. Fems Microbiology Letters, v. 193, p. 249–253, 2000.

WANG, X. P. et al. Study on the bioactivity of the endophytic fungi isolated fromSinopodophyllum hexandrum and Diphylleia sinensis. Natural Product Research and Development, v. 8, p. 15–19, 2006.

WEBER, D. et al. Phomol, a new antinflammatory metabolite from an endophyte of the medicinal plant Erythrina crista-galli. Journal of Antibiotics, v. 57, p. 559–563, 2004.

WEBER, R. W. S. Anti-Candida metabolites from endophytic fungi. Phytochemistry, v. 68, p. 886–892, 2007.

WICKLOW, D. T. et al. A protective endophyte of maize: acremonium zeae antibiotics inhibitory to Aspergillus flavus and Fusarium verticillioides. Mycology Research, v. 109, p. 610–618, 2005.

WU, L. et al. Cytotoxic metabolites from Perenniporia tephropora, an endophytic fungus from Taxus chinensis var. mairei. Applied Microbiology and. Biotechnology, v. 97, p. 305–315, 2013.

WU, Y. Z. et al. Bysspectin A, an unusual octaketide dimer and the precursor derivatives from the endophytic fungus Byssochlamys spectabilis IMM0002 and their biological activities. European Journal of Medicinal Chemistry, v. 145, p. 717-725, 2018.

ZENG, S. R. et al. Isolation of endophytic fungi from Polygonum cuspidatum sieb.et zucc.and screening of isolates producing antibiotic active substances. Journal of Fungal Research, v.3, p. 24–26, 2005.

ZHANG, B. et al. Discovery of a small molecule insulin mimetic with antidiabetic activity in mice. Science, v. 284, p. 974–981,1999.

ZHANG, H. W.; SONG, Y. C.; TAN, R. X. Biology and chemistry of endophytes. Natural Products Report, v.23, p.753–771, 2006.

ZHOU, X. et al. A review: recent advances and future prospects of taxol-producing endophytic fungi. Applied Microbiology and Biotechnology, v.86, p.1707-1717, 2010.

Downloads

Publicado

2021-02-19

Como Citar

Villas Boas, D. F., Luiz, J. H. H., Malpass, G. R. P., Okura, M. H., de Souza, C. P., & Granato, A. C. (2021). Microrganismos endofíticos como fonte de compostos de interesse medicinal – uma breve revisão. Revista Brasileira De Ciência, Tecnologia E Inovação, 5(1), 70–86. https://doi.org/10.18554/rbcti.v5i1.3600

Edição

Seção

Artigos de Revisão