Immunosensor for bovine anaplasmosis diagnosis on graphite platform functionalized with poly (3-hydroxybenzoic acid)

Autores

  • Deusmaque Carneiro Ferreira Departamento de Engenharia Ambiental, Universidade Federal do Triângulo Mineiro (UFTM) http://orcid.org/0000-0001-9338-0863
  • Odonírio Abrahão Júnior Instituto de Ciências Biológicas e Naturais – Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brasil
  • Robson Tadeu Soares de Oliveira Júnior Universidade Federal do Triângulo Mineiro - UFTM
  • Paula de Souza Santos Universidade Federal de Uberlândia - UFU
  • Ana Graci Brito Madurro Universidade Federal de Uberlândia - UFU
  • João Marcos Madurro Universidade Federal de Uberlândia - UFU

DOI:

https://doi.org/10.18554/rbcti.v8i1.6505

Palavras-chave:

Electrochemical, Voltammetry, Immunosensor, Anaplasma marginale, Surface modification

Resumo

This study describes the development of an electrochemical immunosensor with a transducer platform functionalized with the poly-(3- hydroxybenzoic acid), to detection of antibodies against the surface protein (Am1) of Anaplasma marginale . This biological probe was immobilized on the polymer under graphite and characterized electrochemically. The detections of antigen-antibody interactions were conducted using the signal obtained from the oxidation of 4-aminoantipyrine (4 -AAP), interleaved with Am1, to enable the use of voltammetry technique of differential pulse. The detections of antibodies of A. marginable, using the graphite electrode functionalized with the probe Am1 was 66% higher than the graphite electrode not functionalized. The antigen (Am1) interacted with positive serum in dilutions of 1:1000, 1:800, 1:400, 1:200, 1:100, 1:50, and 1:25, demonstrating excellent sensitivity of the immunosensor. The time of storage of the immunosensor was satisfactory, with reduced peak current by 35%, after 90 days. The results demonstrate the excellent applicability of this new functionalized platform for probe immobilization and diagnosis of anaplasmosis without the interference of the most similar bovine diseases.

Biografia do Autor

Deusmaque Carneiro Ferreira, Departamento de Engenharia Ambiental, Universidade Federal do Triângulo Mineiro (UFTM)

Licenciatura em Química pela Universidade de Uberaba, especialista em Química pela Universidade Federal de Lavras, Mestre em Química (Eletroquímica Aplicada) pela Universidade Federal de Uberlândia, Doutor em Química (Eletroquímica Aplicada) pela Universidade Federal de Uberlândia. Graduado em Biomedicina pela Universidade Federal do Triângulo Mineiro. Tem experiência na área de Química com ênfase nos seguintes temas: Produção de biocombustíveis, gestão e gerenciamento de resíduos sólidos, biossorção e dessorção de metais tóxicos, extração e caracterização de produtos naturais de interesse ambiental, desenvolvimento de novas plataformas para imobilização de biomoléculas na superfície transdutora de biossensores. Atualmente é professor Adjunto II da Universidade Federal do Triângulo Mineiro (UFTM), dedicação exclusiva, no departamento de Engenharia Ambiental do Instituto de Ciências Tecnológicas e Exatas (ICTE). Professor permanente e vice-coordenador do Mestrado Acadêmico em Ciência e Tecnologia Ambiental no Programa de Pós-Graduação em Ciência e Tecnologia Ambiental (PPGCTA-UFTM). Atuo no Programa de Mestrado Profissional em Inovação Tecnológica ( PMPIT) da Universidade Federal do Triângulo Mineiro.

Robson Tadeu Soares de Oliveira Júnior, Universidade Federal do Triângulo Mineiro - UFTM

Professor of the Institute of Biological and Natural Sciences, Federal University of the Triângulo Mineiro, Brazil.

Paula de Souza Santos, Universidade Federal de Uberlândia - UFU

Professor of the Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia-MG, Brazil.

Ana Graci Brito Madurro, Universidade Federal de Uberlândia - UFU

Professor of the Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia-MG, Brazil.

João Marcos Madurro, Universidade Federal de Uberlândia - UFU

Instituto de Química, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brasil

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Publicado

2023-10-31

Como Citar

Ferreira, D. C., Abrahão Júnior, O., Oliveira Júnior, R. T. S. de, Souza Santos, P. de, Brito Madurro, A. G. ., & Madurro, J. M. (2023). Immunosensor for bovine anaplasmosis diagnosis on graphite platform functionalized with poly (3-hydroxybenzoic acid). Revista Brasileira De Ciência, Tecnologia E Inovação, 8(1), 14–29. https://doi.org/10.18554/rbcti.v8i1.6505

Edição

v. 8 n. 1 (2023): Janeiro/Junho

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Artigos

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Copyright (c) 2023 Deusmaque Carneiro Ferreira, Odonírio Abrahão Júnior, Robson Tadeu Soares de Oliveira Júnior, Paula de Souza Santos, Ana Graci Brito Madurro, João Marcos Madurro

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